6A56

AJLec from the Sea Anemone Anthopleura japonica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2016-11-16
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1000
Spacegroup name	P 21 21 21
Unit cell lengths	38.079, 79.732, 106.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.080 - 1.200
R-factor	0.14408
Rwork	0.142
R-free	0.17638
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.887
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.700	1.230
High resolution limit [Å]	1.200	1.200
Rmerge	0.056	0.243
Number of reflections	101402
<I/σ(I)>	25.6
Completeness [%]	99.7
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	100 mM lactose, 1 mM CaCl2, 200 mM NaCl, 100 mM HEPES (pH 7.5), 30% polyethylene glycol 3350