6A52
Oxidase ChaP-H1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.610, 49.940, 59.330 |
| Unit cell angles | 90.00, 96.79, 90.00 |
Refinement procedure
| Resolution | 43.304 - 2.000 |
| R-factor | 0.1992 |
| Rwork | 0.194 |
| R-free | 0.25010 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.865 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.900 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.111 | 0.418 |
| Rmeas | 0.157 | 0.590 |
| Rpim | 0.111 | 0.415 |
| Number of reflections | 15603 | 1189 |
| <I/σ(I)> | 4.4 | 1.8 |
| Completeness [%] | 90.6 | |
| Redundancy | 2.1 | |
| CC(1/2) | 0.980 | 0.741 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295.15 | (NH4)2SO4, MES monohydrate, polyethylene glycol monomethyl ether 5000 |
