Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-18 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.860, 78.860, 37.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.760 - 1.750 |
R-factor | 0.17765 |
Rwork | 0.176 |
R-free | 0.21522 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 193l |
RMSD bond length | 0.005 |
RMSD bond angle | 1.056 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.760 | 1.817 |
High resolution limit [Å] | 1.750 | 1.755 |
Number of reflections | 10487 | |
<I/σ(I)> | 12.12 | |
Completeness [%] | 86.2 | |
Redundancy | 2 | |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 300 | The concentration of the protein was 20mg/ml. The precipitant solution in the reservoir was 0.1M sodium acetate/acetic acid buffer of pH 4.6 to 5.0 containing 1.2 to 1.5M sodium chloride. The soaking solution was 0.1M sodium acetate/acetic acid buffer at pH 3.5 containing 1.4M NaCl, 25% v/v glycerol (as cryo-protectant) and 2.5M of guanidine hydrochloride. |