6A4K
Human antibody 32D6 Fab in complex with H1N1 influenza A virus HA1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-12 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 181.743, 181.743, 248.091 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.150 |
R-factor | 0.17792 |
Rwork | 0.175 |
R-free | 0.23287 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gbn 3n9g |
RMSD bond length | 0.010 |
RMSD bond angle | 1.532 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.260 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.077 | 0.554 |
Number of reflections | 79824 | 7965 |
<I/σ(I)> | 14.7 | 2.1 |
Completeness [%] | 98.6 | 99.3 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 14%(w/v) PEG 8000, 160mM Calcium acetate, 20%(v/v) glycerol, 80mM Sodium Cacodylate, Hydrochloric acid |