6A48
Crystal structure of reelin N-terminal region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-02-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.753, 90.999, 90.535 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.267 - 2.000 |
| R-factor | 0.2168 |
| Rwork | 0.215 |
| R-free | 0.25690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e26 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.817 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 46788 | 4744 |
| <I/σ(I)> | 26 | 2.4 |
| Completeness [%] | 97.2 | 99.2 |
| Redundancy | 6.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Tris-HCl (pH 8.0), 0.4M sodium chloride,25% (w/v) PEG monomethyl ether 5000 |
