6A43
R1EN(5-225)-ubiquitin fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2015-07-06 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.900 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 141.460, 141.460, 37.993 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.020 - 2.400 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ei9 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.166 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.174 | 1.498 |
Number of reflections | 17225 | 1775 |
<I/σ(I)> | 10.2 | 1.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.5 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | 2.0M sodium acetate, 10mM ammonium sulfate, 1% Jeffamine M600 |