6A42
R1EN(5-223)-ubiquitin fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2015-06-03 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.900 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 141.206, 141.206, 37.905 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.230 - 1.700 |
R-factor | 0.19245 |
Rwork | 0.191 |
R-free | 0.22001 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ei9 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.595 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.131 | 0.866 |
Rmeas | 0.144 | 0.961 |
Rpim | 0.060 | 0.408 |
Number of reflections | 45813 | 1724 |
<I/σ(I)> | 11.4 | 2.4 |
Completeness [%] | 96.0 | 68.7 |
Redundancy | 11.2 | 10.1 |
CC(1/2) | 0.994 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 283 | 2.0M sodium acetate, 10mM ammonium sulfate, 1% Jeffamine M600, |