6A2U
Crystal structure of gamma-alpha subunit complex from Burkholderia cepacia FAD glucose dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 110.521, 110.521, 524.877 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.740 - 2.600 |
| R-factor | 0.2068 |
| Rwork | 0.204 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | The structure of selenomethionine variant |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.574 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 7.050 | 2.600 |
| Rmerge | 0.150 | 0.120 | 0.428 |
| Rmeas | 0.154 | 0.124 | 0.439 |
| Rpim | 0.034 | 0.029 | 0.094 |
| Total number of observations | 1273970 | ||
| Number of reflections | 60005 | 3400 | 2920 |
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 99.9 | 99.1 | 100 |
| Redundancy | 21.2 | 18.2 | 21.4 |
| CC(1/2) | 0.992 | 0.979 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Tacsimate |
