6A2U

Crystal structure of gamma-alpha subunit complex from Burkholderia cepacia FAD glucose dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-25
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	110.521, 110.521, 524.877
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.740 - 2.600
R-factor	0.2068
Rwork	0.204
R-free	0.26080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	The structure of selenomethionine variant
RMSD bond length	0.002
RMSD bond angle	0.574
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.150	0.120	0.428
Rmeas	0.154	0.124	0.439
Rpim	0.034	0.029	0.094
Total number of observations	1273970
Number of reflections	60005	3400	2920
<I/σ(I)>	7.3
Completeness [%]	99.9	99.1	100
Redundancy	21.2	18.2	21.4
CC(1/2)		0.992	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	Tacsimate