6A2H

Architectural roles of Cren7 in folding crenarchaeal chromatin filament

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	40.642, 40.642, 104.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.2143
Rwork	0.211
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lwh
RMSD bond length	0.012
RMSD bond angle	1.432
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.053	0.996
Number of reflections	4371
<I/σ(I)>	1.9
Completeness [%]	100.0
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M Bis-Tris, pH 6.5, 25% PEG 300