6A2B
Crystal Structure of Xenopus laevis MHC I complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-25 |
| Detector | Nonius Kappa CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.139, 68.220, 107.809 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.800 |
| R-factor | 0.22595 |
| Rwork | 0.200 |
| R-free | 0.26437 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e0r |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.716 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.830 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.188 | |
| Number of reflections | 29548 | 4256 |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 92.5 | 91.7 |
| Redundancy | 2 | |
| CC(1/2) | 0.939 | 0.926 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M sodium malonate pH6.0, 20% w/v PEG 3350 |
