6A27
Crystal structure of PprA W183R mutant form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.459, 99.357, 48.101 |
| Unit cell angles | 90.00, 110.05, 90.00 |
Refinement procedure
| Resolution | 25.720 - 1.353 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.20150 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6a28 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.034 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.720 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.051 | 0.380 |
| Number of reflections | 76916 | 7671 |
| <I/σ(I)> | 33.8 | 2.3 |
| Completeness [%] | 92.4 | 92.6 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris buffer (pH 8.5) containing 0.2 M LiSO4 and 30% PEG4000 |
