6A1X

Charcot-Leyden crystal protein/Galectin-10 variant W127A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 65 2 2
Unit cell lengths	48.502, 48.502, 259.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.472 - 1.990
R-factor	0.1895
Rwork	0.186
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.472	2.040
High resolution limit [Å]	1.990	1.990
Number of reflections	13492
<I/σ(I)>	15.8
Completeness [%]	99.9
Redundancy	17.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Bistris