6A1Q

Crystal structures of disordered Z-type helices

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2009-07-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 32
Unit cell lengths	30.675, 30.675, 42.355
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.560 - 2.501
R-factor	0.2714
Rwork	0.274
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p4j
RMSD bond length	0.014
RMSD bond angle	1.646
Data reduction software	XDS (GPLv2)
Data scaling software	XDS (GPLv2)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	16.560	16.560	2.650
High resolution limit [Å]	2.500	6.900	2.500
Rmerge	0.061	0.057	0.144
Rmeas	0.076	0.071	0.181
Total number of observations	7706
Number of reflections	2962	137	462
<I/σ(I)>	9.13	12.68	4.37
Completeness [%]	95.9	91.3	95.1
Redundancy	2.602	2.584	2.619
CC(1/2)	0.996	0.997	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1mM DNA, 50mM sodium cacodylate trihydrate buffer, 200mM MgSO4, 50% MPD.