6A03

Structure of pSTING complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	50.517, 80.459, 101.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.231 - 2.597
R-factor	0.2057
Rwork	0.200
R-free	0.25720
RMSD bond length	0.003
RMSD bond angle	0.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_3092))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.597	2.600
Number of reflections	13319
<I/σ(I)>	19.85
Completeness [%]	100.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	3.5M sodium formate