5ZYS
Structure of Nephrin/MAGI1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-13 |
Detector | DECTRIS PILATUS3 X 1M |
Wavelength(s) | 0.97853 |
Spacegroup name | I 2 3 |
Unit cell lengths | 93.731, 93.731, 93.731 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.865 - 1.780 |
R-factor | 0.1822 |
Rwork | 0.181 |
R-free | 0.20440 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.173 |
Data reduction software | d*TREK |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
High resolution limit [Å] | 1.780 | 4.830 | 1.780 |
Rmerge | 0.069 | 0.057 | 0.172 |
Rmeas | 0.079 | 0.064 | 0.202 |
Rpim | 0.037 | 0.030 | 0.102 |
Total number of observations | 51381 | ||
Number of reflections | 13086 | 668 | 683 |
<I/σ(I)> | 6.2 | ||
Completeness [%] | 98.1 | 92.8 | 99.9 |
Redundancy | 3.9 | 4 | 3.6 |
CC(1/2) | 0.994 | 0.952 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.5M sodium chloride , 0.01M magnesium chloride hexahydrate , 0.01Mhexadecyltrimethylammonium bromide |