5ZO1

Crystal structure of mouse nectin-like molecule 4 (mNecl-4) full ectodomain (Ig1-Ig3), 2.2A

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-01
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	I 41 2 2
格子定数 [Å]	109.497, 109.497, 182.441
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	47.295 - 2.201
R因子	0.2007
Rwork	0.200
R-free	0.21990
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.783
Data reduction software	XDS
Refinement software	PHENIX ((1.10.1_2155))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	47.295	2.330
分解能 [Å] (高)	2.200	2.200
Rmerge_l_obs	0.144	0.943
独立反射数	28426	8531
<I/σ(I)>	12.15	2.02
完全性 [%]	99.7	98.1
冗長性	6.93	6.79
CC(1/2)	0.996	0.678

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP		291	0.04M Potassium dihydrogen phosphate, 10% PEG 8000, 25% (v/v) Glycerol