5Z83

Crystal structure of GenB1 from Micromonospora echinospora in complex with PLP (internal aldimine)

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-15
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
格子定数 [Å]	77.140, 88.012, 116.140
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	33.798 - 1.700
R因子	0.1591
Rwork	0.158
R-free	0.19230
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.012
結合角の平均二乗偏差(RMSD) [度]	1.190
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2474)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	1.730
分解能 [Å] (高)	1.700	1.700
Rmerge_l_obs	0.055	0.241
Rpim	0.017	0.123
独立反射数	84747	3524
<I/σ(I)>	34
完全性 [%]	97.2
冗長性	9.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	MICROBATCH		295	magnesium formate, PEG3350