5Z6Y

Structure of sfYFP48S95C66BPA

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	200
Detector technology	CCD
Collection date	2017-12-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 41 21 2
格子定数 [Å]	51.574, 51.574, 179.459
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	33.849 - 1.870
R因子	0.21
Rwork	0.209
R-free	0.23580
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.041
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	1.940
分解能 [Å] (高)	1.870	1.870
<I/σ(I)>	15.8
完全性 [%]	100.0
冗長性	12.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	4	289	0.1M sodium malonate pH 4.0, 12% PEG 3350