5Z2C
Crystal structure of ALPK-1 N-terminal domain in complex with ADP-heptose
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2017-02-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | C 1 2 1 |
| 格子定数 [Å] | 169.260, 214.388, 173.151 |
| 格子定数 [度] | 90.00, 110.33, 90.00 |
精密化法
| 残基 | 48.888 - 2.594 |
| R因子 | 0.2422 |
| Rwork | 0.242 |
| R-free | 0.26280 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.599 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 48.890 | 2.660 |
| 分解能 [Å] (高) | 2.590 | 2.590 |
| Rmerge_l_obs | 0.069 | 0.660 |
| 独立反射数 | 174023 | 12581 |
| <I/σ(I)> | 14.62 | 1.99 |
| 完全性 [%] | 96.8 | 95.8 |
| 冗長性 | 3.6 | 3.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 1.4M sodium formate, 0.1M sodium acetate |
