5YVP

Crystal structure of an apo form cyclase Filc1 from Fischerella sp. TAU

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-04
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
格子定数 [Å]	86.758, 255.893, 34.912
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	24.242 - 2.051
R因子	0.214
Rwork	0.212
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yvk
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.109
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Quality characteristics

	Overall	Inner shell	Outer shell
分解能 [Å] (低)	25.000	25.000	2.120
分解能 [Å] (高)	2.050	4.410	2.050
Rmerge_l_obs	0.073	0.052	0.400
Rmeas	0.082	0.059	0.449
Rpim	0.038	0.028	0.201
独立反射数	49655	5160	4856
<I/σ(I)>	15.6
完全性 [%]	99.1	96.3	99.9
冗長性	4.9	4.9	4.9
CC(1/2)		0.994	0.956

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	8	295	1.0M sodium citrate, 0.1M Imidazole pH 8.0