5XLZ

The crystal structure of tubulin complexed with a benzylidene derivative of 9(10H)-anthracenone

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
格子定数 [Å]	105.465, 158.439, 181.600
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	42.668 - 2.298
R因子	0.2165
Rwork	0.216
R-free	0.24660
結合長の平均二乗偏差(RMSD) [Å]	0.003
結合角の平均二乗偏差(RMSD) [度]	0.828
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Quality characteristics

	Overall
分解能 [Å] (低)	50.000
分解能 [Å] (高)	2.298
独立反射数	135594
<I/σ(I)>	15.5
完全性 [%]	99.8
冗長性	6.8

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	6% poly(ethylene glycol) 4000, 8% glycerol, 0.1M Mes, 30mM CaCl2, 30mM MgCl2, pH 6.7