5WTL
Crystal structure of the periplasmic portion of outer membrane protein A (OmpA) from Capnocytophaga gingivalis
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 73.205, 87.343, 185.458 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 42.509 - 2.298 |
| R因子 | 0.2057 |
| Rwork | 0.203 |
| R-free | 0.25510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4erh |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.016 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.072 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 42.509 | 2.340 |
| 分解能 [Å] (高) | 2.298 | 2.300 |
| 独立反射数 | 53087 | |
| <I/σ(I)> | 8.6 | |
| 完全性 [%] | 98.6 | |
| 冗長性 | 3.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100 mM MES, pH 6.0, 200 mM Ca(Ac)2, 20% PEG 8000 |
