5W4Q
Structure of the R18A/E28A mutant of the HIV-1 capsid protein
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033203 |
| Spacegroup name | P 6 |
| 格子定数 [Å] | 92.055, 92.055, 58.253 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 47.030 - 2.285 |
| R因子 | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 4XFX |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.003 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.564 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX ((1.11.1-2575_1692)) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 47.034 | 47.030 | 2.360 |
| 分解能 [Å] (高) | 2.280 | 8.850 | 2.280 |
| Rmerge_l_obs | 0.075 | 0.039 | 0.930 |
| Rmeas | 0.083 | 0.044 | 1.026 |
| Rpim | 0.035 | 0.020 | 0.428 |
| 独立反射数 | 12728 | 1246 | |
| <I/σ(I)> | 13.6 | ||
| 完全性 [%] | 98.6 | 99.6 | 98.1 |
| 冗長性 | 5.6 | 5.3 | 5.6 |
| CC(1/2) | 0.996 | 0.995 | 0.579 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG3350, NaI, Sodium cacodylate, Glycerol |
