5V1T
Crystal structure of Streptococcus suis SuiB bound to precursor peptide SuiA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-07-20 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 69.370, 115.040, 54.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.235 - 2.100 |
R-factor | 0.1926 |
Rwork | 0.189 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5v1q |
RMSD bond length | 0.006 |
RMSD bond angle | 0.942 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.240 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.123 | 0.799 |
Number of reflections | 25903 | |
<I/σ(I)> | 9.3 | 1.9 |
Completeness [%] | 99.3 | 97.1 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 285 | A solution containing 23 mg/mL of His6-tagged SuiB in storage buffer [100 mM HEPES, pH 7.5, 300 mM KCl, 5 mM DTT, 10% (v/v) glycerol] was mixed with a stock solution of SuiA in storage buffer lacking DTT, yielding a final SuiA concentration of 1.9 mM. The resultant solution was incubated for 10 min after which it was combined 1:1 with precipitant solution to form a 4 uL drop. Crystals appeared within 2 days and were fully grown within a week. The precipitant solution was generated by combining 100 mM Bis-Tris, pH 6.0, 200 mM Li2SO4, 27% (w/v) PEG 3350 with 210 mM SAM in water to yield a final SAM concentration of 10.5 mM. |