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5TV7

2.05 Angstrom Resolution Crystal Structure of Peptidoglycan-Binding Protein from Clostridioides difficile in Complex with Glutamine Hydroxamate.

Experimental procedure
実験手法SINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-F
Synchrotron siteAPS
Beamline21-ID-F
Temperature [K]100
Detector technologyCCD
Collection date2014-06-04
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)0.97872
Spacegroup nameC 1 2 1
格子定数 [Å]134.949, 35.008, 101.533
格子定数 [度]90.00, 126.25, 90.00
精密化法
残基29.080 - 2.050
R因子0.17346
Rwork0.172
R-free0.21030
Structure solution methodSAD
結合長の平均二乗偏差(RMSD) [Å]0.008
結合角の平均二乗偏差(RMSD) [度]1.313
Data reduction softwareHKL-3000
Data scaling softwareHKL-3000
Phasing softwarePHENIX
Refinement softwareREFMAC (5.8.0155)
Quality characteristics
 OverallOuter shell
分解能 [Å] (低)29.0802.090
分解能 [Å] (高)2.0502.050
Rmerge_l_obs0.0930.769
独立反射数24487
<I/σ(I)>28.12.06
完全性 [%]100.099.9
冗長性55
CC(1/2)0.769
結晶化条件
結晶ID方法pH温度溶液条件
1VAPOR DIFFUSION, SITTING DROP8.5295Protein: 7.8 mg/ml, 0.25M Sodium chloride, 0.01M Tris HCl (pH 8.3), Screen: JCSG+ (B3), 0.1M Bicine (pH 8.5), 20% (w/v) PEG 6000.

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件を2024-09-18に公開中

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