5TGM
Crystal structure of the S.cerevisiae 80S ribosome in complex with the A-site bound aminoacyl-tRNA analog ACCA-Pro
これはPDB形式変換不可エントリーです。
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 434.730, 286.770, 303.150 |
| 格子定数 [度] | 90.00, 98.87, 90.00 |
精密化法
| 残基 | 148.723 - 3.500 |
| R因子 | 0.2619 |
| Rwork | 0.261 |
| R-free | 0.31190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.024 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.743 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.10.1_2155) |
| Refinement software | PHENIX (1.10.1_2155) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 200.000 | 3.600 | |
| 分解能 [Å] (高) | 3.500 | 150.000 | 3.500 |
| Rmerge_l_obs | 0.614 | 1.443 | |
| 独立反射数 | 918642 | ||
| <I/σ(I)> | 4.94 | 1 | |
| 完全性 [%] | 99.8 | 99.6 | |
| 冗長性 | 5 | ||
| CC(1/2) | 0.988 | 0.363 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG20000 |
