5O5O

X-ray crystal structure of RapZ from Escherichia coli (P32 space group)

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-01
Detector	MARRESEARCH
Wavelength(s)	0.87260
Spacegroup name	P 32
格子定数 [Å]	92.750, 92.750, 156.800
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	46.330 - 3.404
R因子	0.2041
Rwork	0.197
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.011
結合角の平均二乗偏差(RMSD) [度]	1.323
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2747: ???))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	46.330	3.480
分解能 [Å] (高)	3.400	3.400
Rmerge_l_obs	0.123	0.638
独立反射数	20540
<I/σ(I)>	11.2	1.8
完全性 [%]	99.7	99.9
冗長性	3.5	3.58
CC(1/2)	0.995	0.695

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1 M MES, 1.1 M ammonium sulfate, 0.01 M beta-mercaptoethanol, 1 percent glycerol, pH 7.0