5NJ5
E. coli Microcin-processing metalloprotease TldD/E with phosphate bound
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9200 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 64.770, 173.990, 83.520 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 75.290 - 1.900 |
| R因子 | 0.1406 |
| Rwork | 0.139 |
| R-free | 0.17810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tv9 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.010 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.376 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.12) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 75.290 | 75.290 | 1.950 |
| 分解能 [Å] (高) | 1.900 | 8.500 | 1.900 |
| Rmerge_l_obs | 0.087 | 0.027 | 0.551 |
| Rmeas | 0.100 | 0.031 | 0.683 |
| Rpim | 0.047 | 0.014 | 0.392 |
| 独立反射数 | 136171 | ||
| <I/σ(I)> | 12.3 | ||
| 完全性 [%] | 94.1 | 99.1 | 69.1 |
| 冗長性 | 4.2 | 4.6 | 3 |
| CC(1/2) | 0.997 | 0.999 | 0.565 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | NULL |
