5N74

Microtubule end binding protein complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-11
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	42.350, 183.272, 42.387
Unit cell angles	90.00, 112.33, 90.00

Refinement procedure

Resolution	45.820 - 2.300
R-factor	0.23237
Rwork	0.230
R-free	0.27059
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gjo
RMSD bond length	0.008
RMSD bond angle	1.165
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.820	2.430
High resolution limit [Å]	2.300	2.300
Rmerge	0.051	0.504
Rmeas	0.056	0.501
Rpim	0.020	0.208
Number of reflections	52463	8301
<I/σ(I)>	2.8	2.83
Completeness [%]	99.0	97.3
Redundancy	6.8
CC(1/2)		0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	100 mM PCTP buffer, pH 4, supplemented with 25% PEG 1500