5L0C
Human metavinculin (residues 959-1134) in complex with PIP2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.520, 100.676, 105.615 |
Unit cell angles | 90.00, 122.49, 90.00 |
Refinement procedure
Resolution | 30.450 - 3.100 |
R-factor | 0.2368 |
Rwork | 0.235 |
R-free | 0.26060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3myi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.130 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.080 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rpim | 0.034 | |
Number of reflections | 18132 | |
<I/σ(I)> | 15.2 | 2.1 |
Completeness [%] | 98.2 | 100 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.999 | 0.742 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 20 mM citric acid, 80 mM Bis-Tris propane (pH 3.4), and 16% (w/v) PEG 3350 |