5HDG
crystal structure of heat shock factor 1-DBD
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | I 2 2 2 |
| 格子定数 [Å] | 48.610, 64.420, 68.770 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 32.210 - 1.700 |
| R因子 | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hts |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.003 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.753 |
| Data reduction software | XSCALE |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 32.210 | 1.730 |
| 分解能 [Å] (高) | 1.700 | 1.700 |
| Rmerge_l_obs | 0.120 | 0.527 |
| 独立反射数 | 12343 | |
| <I/σ(I)> | 4.1 | 3 |
| 完全性 [%] | 99.5 | 99.8 |
| 冗長性 | 7.1 | 7.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.2M sodium formate, pH 7.2, 22%(w/v) PEG3350 |
