5GZN

Structure of neutralizing antibody bound to Zika envelope protein

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
格子定数 [Å]	124.708, 129.385, 428.711
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	42.080 - 3.000
R因子	0.2243
Rwork	0.223
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jhm 5jhl
結合長の平均二乗偏差(RMSD) [Å]	0.004
結合角の平均二乗偏差(RMSD) [度]	0.847
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	3.110
分解能 [Å] (高)	3.000	3.000
独立反射数	69280
<I/σ(I)>	10.7
完全性 [%]	99.5
冗長性	4.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M potassium chloride, 0.1 M HEPES, 25%(v/v) SOKALAN CP 7