5GGF
Crystal structure of human protein O-mannose beta-1,2-N-acetylglucosaminyltransferase form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.1000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 168.688, 186.444, 143.887 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.611 - 2.490 |
| R-factor | 0.2335 |
| Rwork | 0.233 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.610 | 2.550 |
| High resolution limit [Å] | 2.497 | 2.500 |
| Rmerge | 0.112 | 1.200 |
| Number of reflections | 79199 | |
| <I/σ(I)> | 16.13 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.4-1.6M Na malonate |
