5F1E
Apo protein of Sandercyanin
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 63 2 2 |
| 格子定数 [Å] | 158.764, 158.764, 84.795 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 53.400 - 2.700 |
| R因子 | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f6z |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.088 |
| Data reduction software | iMOSFLM |
| Data scaling software | d*TREK |
| Phasing software | d*TREK |
| Refinement software | PHENIX (1.8.1_1168) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 53.400 |
| 分解能 [Å] (高) | 2.700 |
| 独立反射数 | 17824 |
| <I/σ(I)> | 31.48 |
| 完全性 [%] | 100.0 |
| 冗長性 | 36.7 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M HEPES, 10% PEG 8000, 8% v/v Ethylene glycol |
