5B7Z
Crystal Structure of Hyperthermophilic Thermotoga maritima L-Ketose-3-Epimerase with Ni2+
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 1 |
| 格子定数 [Å] | 50.225, 55.220, 58.693 |
| 格子定数 [度] | 107.16, 102.23, 92.35 |
精密化法
| 残基 | 41.513 - 1.500 |
| R因子 | 0.1735 |
| Rwork | 0.172 |
| R-free | 0.19720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b7y |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.112 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.530 |
| 分解能 [Å] (高) | 1.500 | 1.500 |
| 独立反射数 | 90302 | |
| <I/σ(I)> | 13.27 | 3.13 |
| 完全性 [%] | 96.0 | 92.8 |
| 冗長性 | 3.5 | 3.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100mM MES, 5%(w/w) PEG1000, 20%(v/v) PEG200 |
