5AX0
Crystal Structure of the Cell-Free Synthesized Membrane Protein, Acetabularia Rhodopsin I, at 1.52 angstrom
Replaces: 3WT9Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-27 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 75.990, 101.630, 43.760 |
Unit cell angles | 90.00, 119.17, 90.00 |
Refinement procedure
Resolution | 38.210 - 1.521 |
R-factor | 0.1767 |
Rwork | 0.176 |
R-free | 0.19630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3w |
RMSD bond length | 0.006 |
RMSD bond angle | 0.919 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.210 | 1.580 |
High resolution limit [Å] | 1.520 | 1.520 |
Number of reflections | 44164 | |
<I/σ(I)> | 12.19 | 1.77 |
Completeness [%] | 99.4 | 97.93 |
Redundancy | 3.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | 18% PEG400, 0.1M Hepes/NaOH, 0.2M NaCl, 10mM MgCl2, 2.5% MPD |