5A7Q
Crystal structure of human JMJD2A in complex with compound 30
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 101.194, 149.080, 57.627 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 83.727 - 2.000 |
| Rwork | 0.194 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oq7 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 149.080 | 2.110 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.050 | 0.610 |
| 独立反射数 | 59779 | |
| <I/σ(I)> | 19.2 | 2.3 |
| 完全性 [%] | 99.9 | 99.8 |
| 冗長性 | 5.4 | 4.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 6.5 | 28% PEG3350 -- 0.1M BIS-TRIS PH 6.5 -- 0.15M AMMONIUM SULFATE |
