5ZZV
Crystal structure of PEG-1500 crystallized Peptidyl-tRNA Hydrolase from Acinetobacter baumannii at 1.5 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9095 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 109.025, 34.383, 58.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.930 - 1.570 |
| R-factor | 0.17177 |
| Rwork | 0.170 |
| R-free | 0.20544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fop |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.888 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.930 | 1.630 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmeas | 0.145 | 0.145 |
| Number of reflections | 24836 | 24836 |
| <I/σ(I)> | 8.7 | 8.7 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 12 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM HEPES, 15% PEG 1500 |
