5ZZU
Crystal structure of the C-terminal periplasmic domain of EcEptC from Escherichia coli- complex with Zn
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-20 |
Detector | DECTRIS PILATUS3 R 1M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 32 |
Unit cell lengths | 85.060, 85.060, 121.340 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.830 - 2.100 |
R-factor | 0.1662 |
Rwork | 0.165 |
R-free | 0.19730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ZNV |
RMSD bond length | 0.005 |
RMSD bond angle | 0.776 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXS |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.830 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.089 | 0.427 |
Rmeas | 0.094 | 0.472 |
Rpim | 0.030 | 0.196 |
Number of reflections | 56697 | 5219 |
<I/σ(I)> | 17.6 | 3.1 |
Completeness [%] | 98.7 | 90.11 |
Redundancy | 9.5 | 5.48 |
CC(1/2) | 0.999 | 0.887 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M Ammonium formate (pH7.5), 20% (w/v) PEG3350 |