5ZZN

Crystal structure of photosystem II from an SQDG-deficient mutant of Thermosynechococcus elongatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	122.250, 228.430, 287.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 2.100
R-factor	0.1562
Rwork	0.154
R-free	0.19660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wu2
RMSD bond length	0.021
RMSD bond angle	2.582
Data reduction software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.990	2.220
High resolution limit [Å]	2.100	2.100
Rmerge	0.087	0.886
Number of reflections	459862	73153
<I/σ(I)>	13.7	2.1
Completeness [%]	99.5	98.5
Redundancy	6.8	6.7
CC(1/2)	0.998	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6	285	5% PEG 1450, 20MM NACL, 10MM CACL2, 40MM MGSO4, 20MM MES BUFFER, PH 6.0, MICRO-BATCH METHOD UNDER OIL, TEMPERATURE 285K