5ZZB

LokiProfilin2/Rabbit Actin Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-17
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	53.850, 123.300, 88.240
Unit cell angles	90.00, 96.90, 90.00

Refinement procedure

Resolution	61.650 - 2.300
R-factor	0.22993
Rwork	0.228
R-free	0.27448
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yee
RMSD bond length	0.010
RMSD bond angle	1.484
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.700	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.117	0.292
Rmeas	0.139	0.346
Rpim	0.073	0.185
Number of reflections	50253	2600
<I/σ(I)>	10.9	3.4
Completeness [%]	99.3	97.2
Redundancy	3.5	3.4
CC(1/2)	0.961	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	100 mM PCTP 25% PEG1500