5ZYW

The crystal structure of apo-HsMGME1 with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 43 21 2
Unit cell lengths	67.997, 67.997, 114.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.950 - 2.200
R-factor	0.22735
Rwork	0.226
R-free	0.25234
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zyt
RMSD bond length	0.007
RMSD bond angle	1.113
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.105
Number of reflections	14320	1388
<I/σ(I)>	31
Completeness [%]	100.0
Redundancy	21.6
CC(1/2)		0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.35 M di-sodium tartrate, 21% PEG 3350 and 0.1 M MnCl2