5ZYV

Crystal structure of human MGME1 with single strand DNA2 and Ca2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	81.282, 106.151, 33.391
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.300 - 2.720
R-factor	0.2146
Rwork	0.212
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zyt
RMSD bond length	0.004
RMSD bond angle	0.613
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.095
Number of reflections	8086	708
<I/σ(I)>	17.3
Completeness [%]	97.6
Redundancy	7.5
CC(1/2)		0.565

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1M CHESpH 9.5 and 20% PEG 8000