5ZYU

The crystal structure of humanMGME1 with single strand DNA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-18
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 32
Unit cell lengths	80.714, 80.714, 79.769
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.368 - 1.752
R-factor	0.1749
Rwork	0.173
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zyt
RMSD bond length	0.011
RMSD bond angle	1.036
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.810
High resolution limit [Å]	1.750	1.750
Rmerge	0.094
Number of reflections	58405	5780
<I/σ(I)>	16.4
Completeness [%]	100.0
Redundancy	9.6
CC(1/2)		0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	0.1 M Phosphoric-citric acid pH 4.2 and 20% PEG300