5ZYQ
The Structure of Human PAF1/CTR9 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 82.415, 82.415, 145.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.207 - 2.531 |
| R-factor | 0.2037 |
| Rwork | 0.202 |
| R-free | 0.24020 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.078 |
| Data reduction software | HKL-2000 (v714) |
| Data scaling software | HKL-2000 (v714) |
| Phasing software | PHENIX ((1.10_2155_000)) |
| Refinement software | PHENIX ((1.10_2155_000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rpim | 0.022 | 0.208 |
| Number of reflections | 17813 | 964 |
| <I/σ(I)> | 28.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1M Tris-HCl, pH 8.0, 27.5% PEG 400, 6.875% MPD |
