5ZYC
Crystal Structure of Glucose Isomerase Soaked with Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-28 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.987 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.456, 97.921, 101.988 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.630 - 1.750 |
| R-factor | 0.16164 |
| Rwork | 0.161 |
| R-free | 0.18411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y4i |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.750 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.630 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.110 | 0.364 |
| Rmeas | 0.126 | 0.414 |
| Rpim | 0.059 | 0.059 |
| Number of reflections | 46646 | 2302 |
| <I/σ(I)> | 20.69 | |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 4.4 | 4.4 |
| CC(1/2) | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.5 | PEG 400, MgCl2 |
