5ZXL
Structure of GldA from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 190 |
| Detector technology | CCD |
| Collection date | 2017-04-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 162.446, 162.446, 293.127 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.611 - 2.794 |
| R-factor | 0.1741 |
| Rwork | 0.173 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mca |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.978 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.611 | 2.894 |
| High resolution limit [Å] | 2.790 | 2.794 |
| Rmerge | 0.095 | 0.722 |
| Number of reflections | 97055 | 9400 |
| <I/σ(I)> | 14.83 | |
| Completeness [%] | 60.0 | |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 0.02M Tris-HCl(pH7.4), 0.05mM sodium chloride, 0.15mM ammonium acetate |
