5ZX1

Crystal structure of ENT domain from T. brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2018-05-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	I 41 2 2
Unit cell lengths	132.590, 132.590, 40.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.878 - 2.310
Rwork	0.234
R-free	0.27880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uz3
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX (1.13-2998)
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.880	2.390
High resolution limit [Å]	2.310	2.310
Rmerge	0.028	0.270
Rpim	0.028
Number of reflections	8072	1586
<I/σ(I)>	14.5
Completeness [%]	99.0
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2M L-proline, 0.1M HEPES pH7.5, 10% PEG 3350