5ZWU
Crystal structure of recombination mediator protein RecF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 37.700, 74.380, 153.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.130 - 1.580 |
| R-factor | 0.17256 |
| Rwork | 0.170 |
| R-free | 0.22040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o5v |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.856 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.130 | 1.600 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.044 | 0.355 |
| Number of reflections | 60237 | |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 99.3 | |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 0.1M Sodium malonate pH 7.0, 0.1M HEPES pH 7.0, 0.5 % v/v Jeffamine ED-2001 |
