5ZWP
Crystal structure of the delta-class glutathione transferase from Musca domestica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.260, 90.909, 51.418 |
| Unit cell angles | 90.00, 107.56, 90.00 |
Refinement procedure
| Resolution | 49.030 - 1.400 |
| R-factor | 0.15773 |
| Rwork | 0.157 |
| R-free | 0.17605 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jlv |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.912 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.030 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.045 | 0.115 |
| Number of reflections | 84246 | 11834 |
| <I/σ(I)> | 8.2 | 5.9 |
| Completeness [%] | 95.9 | 95.9 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M sodium citrate, pH 5.6, 1.8 M ammonium sulfate, 0.1 M tartrate |
